EasyPure® EndoLow Plasmid MaxiPrep Kit
Cat# EM122-01
Size : 10rxns
Brand : TransGen Biotech
ManualEasyPure® EndoFree Plasmid MaxiPrep Kit uses alkaline lysis method to extract plasmid DNA from no more than 500 ml of LB E.
coli culture. Unique silica membrane adsorption technique to efficiently bind plasmid DNA. The solution contains indicators that
can indicate whether the lysis and neutralization are complete through the change of color, so as to visualize the procedure.
Endotoxin is removed on the column, which is fast and simple to use. The purified DNA is suitable for a variety of molecular
biology applications including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection.
Features
• Visualized operation: solution LB (blue) indicates whether the lysis and neutralization are complete through the change of color, so
as to ensure the quality of plasmid extraction.
• Fast: the whole procedure can be performed within one hour.
• Easy to use: on-column rapid endotoxin removal.
• High yield (up to 4 mg nucleic acid load in the purification column).
Storage
at room temperature (15-25 ℃) under dry conditions for one year.
Shipping
at room temperature.
| Component | EM122-01(10 rxns) |
| Resuspension Buffer (RB) | 120 ml |
| Lysis Buffer (LB, Blue) | 120 ml |
| Neutralization Buffer 1 (NB1) | 120 ml |
| Activation Buffer (AB) | 55 ml |
| Wash Buffer (WB) | 25 ml |
| Elution Buffer (EB) | 25 ml |
| RNase A (10 mg/ml ) | 1.2 ml |
| Maxi-Plasmid Spin Column with Collection Tube | 10 each |
| Push Filter | 10 each |
| 50 ml Collection Tube | 10 each |