EasyPure® Bacteria Genomic DNA Kit (with RNase A）
Cat# EE161-01
Size : 50rxns
Brand : TransGen Biotech
ManualEasyPure® Bacteria Genomic DNA Kit uses lysozyme and moderate lysis buffer to lyse cells. Proteinase K is used for protein digestion and RNase A used for RNA digestion. DNA is specifically bound to silica-based column in hypersaline condition, and DNA is eluted by low salt and high pH solution. This kit is suitable for isolating high quality genomic DNA from Gram-positive and Gram-negative bacteria. The isolated DNA is suitable for PCR, restriction enzyme digestion, and Southern blot.
• Fast: the whole process can be completed in 50 minutes.
• High yield: DNA yield up to 20 μg.
Applications
PCR, restriction enzyme digestion and Southern blot
Storage
at room temperature (15-25°C) in a dry place for one year.
Shipping
at room temperature.
| Component | EE161-01 (50 rxns) | EE161-11 (50 rxns) |
| Resuspension Buffer11 (RB11) | 12 ml | 12 ml |
| Lysis Buffer11 (LB11) | 6 ml | 6 ml |
| Binding Buffer11 (BB11) | 10 ml | 10 ml |
| Clean Buffer 11 (CB11) | 55 ml | 55 ml |
| Wash Buffer 11 (WB11) | 12 ml | 12 ml |
| Elution Buffer (EB) | 25 ml | 25 ml |
| RNase A (10 mg/ml) | 1 ml | 0 |
| Proteinase K (20 mg/ml) | 1 ml | 1 ml |
| Genomic Spin Columns with Collection Tubes | 50 each | 50 each |
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