DNase I
Cat# EZ0018
Size : 1mL(10mg/mL)>2.000Ukunitz/mg
Brand : Canvax Biotech
DNase I (10mg/mL) >2.000U kunitz/mg
High Quality & Proven Performance in RNA In Vitro Transcription Reactions
DNase I is a recombinant endonuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, to release di-, tri- and oligonucleotide products (on average producing tetranucleotides) with 5’-phosphorylated and 3’-hydroxylated ends.
It acts on single-stranded DNA, double-stranded DNA, RNA-DNA hybrids and chromatin. DNase I requires bivalent cations (Mg²⁺ and Ca²⁺) for maximal activity.
GMP-grade reagent also available. Learn more.
Detailed information:
Advantages & Features
- Recombinant: bovine Pancreatic, purified from E. coli (29 kDa monomer).
- High specific activity: more than 2,000 Kunitz units per mg protein.
- High Quality: free of RNase and Proteinase contamination.
- Complete solution: supplied with 10x Reaction Buffer.
Specifications
Unit definition:
One Kunitz unit is defined as the amount of enzyme required for the complete degradation of 1 µg of plasmid DNA in 10 minutes at 37 ˚C.
Note: One Kunitz unit equals to 50 Worthington units.
Includes
– 1 mL DNase I (10 mg/mL)
– 1 mL Reaction Buffer (10x)
* 10x Reaction Buffer: 100 mM Tris-HCl (pH 7.5 at 25°C), 25 mM MgCl2, 1 mM CaCl2
Download documentation
Datasheet MSDSApplications
- Removal of residual genomic DNA from RNA samples.
- Degradation of DNA template in transcription reactions.
- DNAse I footprinting.
- Perform Nick Translation.
Tables & Figures
Quality Control
- Functionally tested for digestion of template DNA after in vitro transcription.
- Confirmed absence of RNAse activity.
- Specific activity assayed by degradation of 1 µg of pUC18 in 40 mM Tris‐HCl (pH 8.0), 10 mM MgSO4, 1 mM CaCl2.
Advice
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
- Rozpędek-Kamińska, W., Piotrzkowska, D., Galita, G., Pytel, D., Kucharska, E., Dziki, Ł., … & Majsterek, I. (2022). Niskocząsteczkowe inhibitory zależnego od PERK szlaku sygnalizacyjnego adaptacyjnej odpowiedzi na stres w celowanej terapii raka jelita grubego. Polski Przegląd Chirurgiczny, 94(6).
- Beatriz, M., Vilaça, R., Anjo, S. I., Manadas, B., Januário, C., Rego, A. C., & Lopes, C. (2022). Defective mitochondrial-lysosomal axis promotes extracellular vesicles release of mitochondrial components in Huntington’s Disease. bioRxiv.
- Beatriz, M., Vilaça, R., Anjo, S. I., Manadas, B., Januário, C., Rego, A. C., & Lopes, C. (2022). Defective mitochondria‐lysosomal axis enhances the release of extracellular vesicles containing mitochondrial DNA and proteins in Huntington’s disease. Journal of Extracellular Biology, 1(10), e65.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.