The DNA Damage (8-OHdG) ELISA BioAssay™ Kit is a competitive assy for the qualification of 8-OHdG in serum, plasma, urine, culture media, cell lysates, tissue samples and saliva. This BioAssay™ kit offers a quick incubation time, stable reagents, and a user-friendly protocol. Analyze results using an absorbance plate reader.||Detection Range:|0.94-60ng/ml||Sensitivity:|0.59ng/ml||Precision: |Intra-assay CV: <5%|Inter-assay CV: <5%||Test Principle:|This ELISA kit is a competitive assay that can be used for the quantification of 8-OHdG in serum, plasma, urine, culture media, cell lysates, tissue samples and saliva. The ELISA utilizes an 8-hydroxy-2-deoxyguanosine-coated plate and an HRP-conjugated 8-OHdG specific detection antibody. This produces an assay with a useful range of 0.94-60ng/ml, and a sensitivity of 0.59 ng/mL. Other highlights of this kit include a quick incubation time (60 minutes), stable reagents, and a user-friendly protocol.||It is important to note that the 8-OHdG antibody used in this assay recognizes both free 8-OHdG and DNA-incorporated 8-OHdG. Since complex samples such as plasma, cell lysates, and tissues are comprised of mixtures of DNA fragments and free 8-OHdG, concentrations of 8-OHdG reported by ELISA methodology will not coincide with those reported by LC-MS where the single nucleoside is typically measured. This shouldbe kept in mind when analyzing and interpreting experimental results.||Kit Components:|474005A: Microtiter Plate, 1x96wells|*474005B: 8-OHdG Standard (3.06ug/ml), 1x100ul|474005C: 8-OHdG (HRP) Mab Antibody, 1x75ul|474005D: Sample and Standard Diluent, 1x50ml|474005E: 8-OhdG Antibody Diluent, 1x13ml|474005F: Wash Buffer Concentrate, 10X, 1x50ml|474005G: TMB Substrate, 1x13ml|474005H: Stop Solution (1M HCl), 1x13ml||Storage and Stability:|Store *474005B powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.