Reagents and instruments for immunology, cell biology and molecular biology.

DFF, NT, Blocking Peptide

Print

Cat# D3224-25P-50ug

Size : 50ug

Brand : US Biological

Request more information

D3224-25P DFF, NT, Blocking Peptide

Clone Type

Polyclonal

Grade

Purified

Shipping Temp

Blue Ice

Storage Temp

-20°C

Peptide corresponding to amino acids 2 to 21 of human DFF45 (1). ||Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45kD DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis (1). Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD (2,3). Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei (2-5). Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.

Applications

Source: Synthetic peptide|Purity: Purified|Concentration: ~0.2mg/ml|Form: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.

Purity

Purified

References

1. Liu X, Zou H, Slaughter C, Wang X. DFF, a heterodimeric protein that functions downstream of caspase-3 to trigger DNA fragmentation during apoptosis. Cell 1997;89:175-184 ||2. Enari M, Sakahira H, Yokoyama H, Okawa K, Iwamatsu A, Nagata S. A caspase-activated DNase that degrades DNA during apoptosis, and its inhibitor ICAD. Nature 1998;391:43-50 ||3. Sakahira H, Enari M, Nagata S. Cleavage of CAD inhibitor in CAD activation and DNA degradation during apoptosis. Nature 1998;391:96-99 ||4. Liu X, Li P, Widlak P, Zou H, Luo X, Garrard WT, Wang X. The 40-kDa subunit of DNA fragmentation factor induces DNA fragmentation and chromatin condensation during apoptosis. Proc Natl Acad Sci USA 1998;95:8461-6 ||5. Mukae N, Enari M, Sakahira H, Fukuda Y, Inazawa J, Toh H, Nagata S. Molecular cloning and characterization of human caspase-activated DNase. Proc Natl Acad Sci USA 1998;95:9123-8 |