DEAE SepFast 6HF
Cat# 452101-100ML
Size : 100ml
Brand : BioToolomics
Introduction
Q SepFast 6HF, DEAE SepFast 6HF, SP SepFast 6HF and CM SepFast 6HF are a group of strong anion exchange, weak anion exchange, strong cation exchange and weak cation exchange media, respectively.
The above ion-exchange chromatography (IEX) media is designed for the general purification of biological molecules in which impure components can be chromatographically separated from target molecules at a less demanding process.
The base matrix is made of highly cross-linked 6% agarose. It is very stable to most of the chemical conditions experienced in the bioprocessing industry. The IEX SepFast 6HF could be a good alternative to the IEX Sepharose 6FF with the benefit of reduced cost. We provide the regulatory support files of individual resins for those cGMP applications.
The core advantages to large-scale biomanufacturings are:
- Good dynamic binding capacity
- Good pressure / flow property
- Low cost
Characteristics of IEX SepFast 6HF
Q SepFast 6HF | DEAE SepFast 6HF | SP SepFast 6HF | CM SepFast 6HF | |
Matrix | Highly cross-linked 6% agarose | |||
Functionalgroup | Quaternary ammonium strong anion | Diethyl-aminoethyl weak anion | Sulfo strong cation | Carboxy- methyl weak cation |
Total ionic capacity | 0.09-0.15 mmol/ml | 0.08-0.13 mmol/ml | 0.09-0.15 mmol/ml | 0.08-0.13 mmol/ml |
Particle size | 50 – 150 μm | |||
Pressure-flow property* | >500 cm/h | |||
Operational pressure | Up to 3 bar | |||
pH stability | 2-14 (short term) and 3-12 (long term) | |||
Working temperature | 4oC – 30oC | |||
Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol | |||
Total ionic capacity | Oxidizing agents, anionic detergents | Oxidizing agents, cationic detergents | ||
Storage | 20% ethanol | 20% ethanol | 20% ethanol + 0.2M sodium acetate | 20% ethanol |
*Measured in a 32 mm ID column at a bed height of 15 cm.