HY-100714A-100mg | D-AP5 [79055-68-8] Biotrend USA

Description

D-AP5 (D-APV) is a selective and competitive NMDA receptor antagonist with a K_d of 1.4 μM. D-AP5 (D-APV) inhibits the glutamate binding site of NMDA receptors^[1]^[2].

IC₅₀ & Target

NMDA Receptor

In Vivo

D-AP5 is a NMDA receptor antagonist. Chronic intraventricular infusion of D-AP5 causes a parallel dose-dependent impairment of spatial learning and long-term potentiation (LTP) in vivo. Intracerebral concentrations of D-AP5 fail to induce any measurable sensorimotor disturbance when spatial learning is prevented^[2].
D-AP5 infusion is associated with a progressive reduction in swim speed over trials. D-AP5 causes sensorimotor disturbances in the spatial task, but these gradually worsened as the animals fail to learn. Rats treated with D-AP5 show a delay-dependent deficit in spatial memory in the delayed matching-to-place protocol for the water maze^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

197.13

Formula

C₅H₁₂NO₅P

CAS No.

79055-68-8

Appearance

Solid

Color

White to off-white

SMILES

N[C@H](CCCP(O)(O)=O)C(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

H₂O : 2 mg/mL (10.15 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Ethanol : < 1 mg/mL (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.0728 mL	25.3640 mL	50.7279 mL
5 mM	1.0146 mL	5.0728 mL	10.1456 mL
10 mM	0.5073 mL	2.5364 mL	5.0728 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (507.28 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.92%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (245 KB) HNMR (246 KB) RP-HPLC (205 KB) OR (249 KB)

Handling Instructions (2659 KB)

References

[1]. R H Evans, et al. The effects of a series of omega-phosphonic alpha-carboxylic amino acids on electrically evoked and excitant amino acid-induced responses in isolated spinal cord preparations. Br J Pharmacol. 1982 Jan;75(1):65-75. [Content Brief]

[2]. Davis S, et al. The NMDA receptor antagonist D-2-amino-5-phosphonopentanoate (D-AP5) impairs spatial learning and LTP in vivo at intracerebral concentrations comparable to those that block LTP in vitro. J Neurosci. 1992 Jan;12(1):21-34. [Content Brief]

[3]. Morris RG, et al. N-methyl-d-aspartate receptors, learning and memory: chronic intraventricular infusion of the NMDA receptor antagonist d-AP5 interacts directly with the neural mechanisms of spatial learning. Eur J Neurosci. 2013 Mar;37(5):700-17. [Content Brief]

Animal Administration ^[1]	Male Lister hooded rats are used in this study. They are housed individually and have free access to food and water at all times except for 12 hr prior to surgery. Animals are implanted with 30 mM D-AP5 (N=6) and tested in the water maze^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. R H Evans, et al. The effects of a series of omega-phosphonic alpha-carboxylic amino acids on electrically evoked and excitant amino acid-induced responses in isolated spinal cord preparations. Br J Pharmacol. 1982 Jan;75(1):65-75. [Content Brief] [2]. Davis S, et al. The NMDA receptor antagonist D-2-amino-5-phosphonopentanoate (D-AP5) impairs spatial learning and LTP in vivo at intracerebral concentrations comparable to those that block LTP in vitro. J Neurosci. 1992 Jan;12(1):21-34. [Content Brief] [3]. Morris RG, et al. N-methyl-d-aspartate receptors, learning and memory: chronic intraventricular infusion of the NMDA receptor antagonist d-AP5 interacts directly with the neural mechanisms of spatial learning. Eur J Neurosci. 2013 Mar;37(5):700-17. [Content Brief]

Animal Administration
^[1]

Male Lister hooded rats are used in this study. They are housed individually and have free access to food and water at all times except for 12 hr prior to surgery. Animals are implanted with 30 mM D-AP5 (N=6) and tested in the water maze^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. R H Evans, et al. The effects of a series of omega-phosphonic alpha-carboxylic amino acids on electrically evoked and excitant amino acid-induced responses in isolated spinal cord preparations. Br J Pharmacol. 1982 Jan;75(1):65-75. [Content Brief]

[2]. Davis S, et al. The NMDA receptor antagonist D-2-amino-5-phosphonopentanoate (D-AP5) impairs spatial learning and LTP in vivo at intracerebral concentrations comparable to those that block LTP in vitro. J Neurosci. 1992 Jan;12(1):21-34. [Content Brief]

[3]. Morris RG, et al. N-methyl-d-aspartate receptors, learning and memory: chronic intraventricular infusion of the NMDA receptor antagonist d-AP5 interacts directly with the neural mechanisms of spatial learning. Eur J Neurosci. 2013 Mar;37(5):700-17. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	5.0728 mL	25.3640 mL	50.7279 mL	126.8199 mL
	5 mM	1.0146 mL	5.0728 mL	10.1456 mL	25.3640 mL
	10 mM	0.5073 mL	2.5364 mL	5.0728 mL	12.6820 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

D-AP5 Related Classifications

Membrane Transporter/Ion Channel Neuronal Signaling
iGluR

D-AP5 [79055-68-8]

Cat# HY-100714A-100mg

Brand : MedChemExpress

Other Forms of D-AP5:

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Complete Stock Solution Preparation Table

D-AP5 Related Classifications

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