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Western Blot (Recombinant protein)
Application

Western Blot (Recombinant protein)

Western blot using CXCL10 polyclonal antibody (Cat # PAB16918) shows detection of recombinant bovine CXCL10 at 9.3 KDa (arrowhead) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1 : 10), and probed with 1 ug/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800™ Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against full length recombinant CXCL10.

    Immunogen

    Recombinant protein corresponding to 83 amino acids of bovine CXCL10.

    Host

    Rabbit

    Reactivity

    Bovine, Goat, Sheep

    Form

    Lyophilized

    Recommend Usage

    ELISA (1:500)
    Western Blot (1 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)

    Storage Instruction

    Store at 4°C on dry atmosphere.
    After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Recombinant protein)

    Western blot using CXCL10 polyclonal antibody (Cat # PAB16918) shows detection of recombinant bovine CXCL10 at 9.3 KDa (arrowhead) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA (BSA-30, diluted 1 : 10), and probed with 1 ug/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800™ Conjugated Goat Anti-Rabbit IgG for 45 min at room temperature.

    Enzyme-linked Immunoabsorbent Assay

  • Publication Reference