Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

The whole cell lysate derived from LNCaP was immunoblotted by CSF1R polyclonal antibody (Cat # PAB12674) at 1 : 500. A major immunoreactive band is observed around ~130kDa (lane 1). This band is blocked by pre-incubation of immunizing peptide (lane 2).

View product citations for antibody PAB12674 on CiteAb
  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic peptide of CSF1R.PAB,polyclonal Ab,polyclonal antibody

    Immunogen

    A synthetic peptide corresponding to C-terminus of human CSF1R.

    Host

    Rabbit

    Theoretical MW (kDa)

    130

    Reactivity

    Human, Mouse, Rat

    Specificity

    This antibody recognizes ~130 KDa of human CSF1R.

    Form

    Liquid

    Purification

    Site-modified epitope affinity purification

    Quality Control Testing

    Antibody Reactive Against Synthetic Peptide.

    Recommend Usage

    ELISA (0.01-0.1 ug/mL)
    Immunohistochemistry (2-5 ug/mL)
    Immunoprecipitation (2-5 ug/mL)
    Western Blot (0.1-1 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS, pH 7.2 (Antibody Stabilizer).

    Storage Instruction

    Store at 4°C for three months. For long term storage store at -20°C to -80°C.
    Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot (Cell lysate)

    The whole cell lysate derived from LNCaP was immunoblotted by CSF1R polyclonal antibody (Cat # PAB12674) at 1 : 500. A major immunoreactive band is observed around ~130kDa (lane 1). This band is blocked by pre-incubation of immunizing peptide (lane 2).

    Immunohistochemistry

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — CSF1R

    Entrez GeneID

    1436

    Gene Name

    CSF1R

    Gene Alias

    C-FMS, CD115, CSFR, FIM2, FMS

    Gene Description

    colony stimulating factor 1 receptor

    Omim ID

    164770

    Gene Ontology

    Hyperlink

    Gene Summary

    The protein encoded by this gene is the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. This receptor mediates most if not all of the biological effects of this cytokine. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in this gene have been associated with a predisposition to myeloid malignancy. The first intron of this gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. [provided by RefSeq

    Other Designations

    CD115 antigen|FMS proto-oncogene|McDonough feline sarcoma viral (v-fms) oncogene homolog|macrophage colony stimulating factor I receptor|soluble CSF1R variant 1

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