COX 1 (Cyclooxygenase-1, PGHS-1, Prostaglandin Endoperoxide Synthase-1, PHS 1, Ovarian Cancer Marker) (Biotin)

Cat# C7904-70F-Biotin-100ul

Size : 100ul

Brand : US Biological

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C7904-70F-Biotin COX 1 (Cyclooxygenase-1, PGHS-1, Prostaglandin Endoperoxide Synthase-1, PHS 1, Ovarian Cancer Marker) (Biotin)

Clone Type
Polyclonal
Host
mouse
Isotype
IgG2b,k
Grade
Affinity Purified
Applications
E IF IHC WB
Crossreactivity
Hu Mo Rt Sh
Shipping Temp
Blue Ice
Storage Temp
-20°C

Cyclooxygenases 1 and 2 (COX-1, COX-2) are enzymes involved in the conversion of arichidonate to prostaglandins. COX-1 and COX-2 are very similar in structure and function, though they vary in expression. COX-1 is normally expressed in most cell types, whereas COX-2 expression is at low levels unless induced by hormonal stimuli. The apparent molecular weight of COX-1 appears to be around 72kD.||The prostanoid family includes PGD2, PGE2, PGF2alpha, PGI2, thromboxane A2 and prostaglandins. The prostaglandins (PGs) are implicated in various physiological and pathophysiological events, including male fertility, menstruation, ovulation, pregnancy, implantation and inflamatory and neoplastic diseases. The biosynthesis of PGs and some other prostanoids, is catalyzed in a rate limiting step by PG-H synthase (also known as cyclooxygenase (COX), PG-endoperoxidase synthase (PTGS)) which converts arachiodonic acid to prostaglandin/prostanoid precursor PGH2. Two cyclooxygenase isozymes, COX1 (human, 576aa, 69-72kD; chromosome 9) and COX2 (human, 604aa, 74kD; chromosome 1) have been identified. COX1, a constitutively expressed isoform, produces physiologically relevant prostanoids such as those in stomach and platelets. COX2 isoform is inducible and rapidly upregulated at inflamation sites and forms proinflamatory prostanoids. The overexpression of COX-2 also leads to tumerogenesis. Recently, a third isoform COX3 (canine 633aa; ~65kD in human aorta) has been reported. Two smaller COX1-derived proteins (partial COX1) PCOX1a (canine 414aa, ~53kD in human aorta) and PCOX1b have also been characterized. The COX3, but not PCOX1a, possesses glycosylation dependent cyclooxygenase activity. The nonsteroidal antiinflammatory drugs (NSAIDs) reduce the formation of prostaglandins by inhibiting the activity of cyclooxygenases (COX1, COX2 and COX3), This ability was associated with inhibition of COX, which converts arachidonic acid to the prostaglandin precursor prostaglandin H2.||Applications: |Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications have not been tested.||Recommended Dilution:|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2b,k|Clone No: 6A52|Host: mouse|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.|Purity: Purified by Protein A affinity chromatography|Immunogen: Ram testes|Specificity: Recognizes COX-1at 72kD. Crossreactivity: to a moderate degree with ovine COX-2, and minimally with human COX-2. Species Crossreactivity: sheep, human, mouse and rat.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Ram testes
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography
Specificity
Recognizes COX-1at 72kD. Crossreactivity: to a moderate degree with ovine COX-2, and minimally with human COX-2. Species Crossreactivity: sheep, human, mouse and rat.
References
1. Charpigny, et al. Endocrinology 138(5):2163-2171, 1997. 2. Ballif, et al. PNAS 93:5544-5549, 1996.|