Reagents and instruments for immunology, cell biology and molecular biology.

Citrate Synthase(CS) Activity Assay Kit

Print

Cat# E-BC-K178-M-48T

Size : 48T

Brand : Elabscience

Request more information

Detection Principle	Citrate synthetase (CS) catalyzes acetyl-CoA and oxaloacetate to produce limonyl-CoA, which can further produce CoA. The yellow product generated by this reaction has a characteristic absorption peak at 412 nm. The activity of CS can be calculated by measuring the change of absorbance value at 412 nm.
Synonyms	CS
Sample Type	Animal tissue,Plant tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (402-422 nm, optimum wavelength: 412 nm)
Research Area	Mitochondria Function , Tricarboxylic Acid（TCA）Cycle , Energy Metabolism
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	2.09 U/L
Detection Range	2.09-110 U/L
Precision	inter-assay CV: 7.9% \| intra-assay CV: 0.05
Assay Time	30 min

Cat.No.	Product Name	Sizes
E-BC-K351-M	Citric Acid (CA) Colorimetric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Rat kidney tissue homogenate	1
10% Rat spleen tissue homogenate	1
10% Rat liver tissue homogenate	1
10% Rat heart tissue homogenate	1
10% Mouse liver tissue homogenate	1
10% Mouse lung tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.