Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)

Cat# E-BC-K125-M-96T

Size : 96T

Brand : Elabscience

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Detection Principle Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA), CoA can combine with the 4,4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.
Synonyms ChAT
Sample Type Animal tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (324 nm)
Research Area Amino Acids And Proteins
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period 12 months
Sensitivity 1.21 U/g fresh weigh
Detection Range 1.21-40 U/g fresh we
Precision inter-assay CV: 9.4% | intra-assay CV: 5.1%
Assay Time 1 h 10 min
Cat.No. Product Name Sizes
E-BC-K125-S Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples) 100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
20% Mouse brain tissue homogenate 1-2
20% Rat heart tissue homogenate 1
20% Rat liver tissue homogenate 1
20% Mouse kidney tissue homogenate 1
20% Rat lung tissue homogenate 1
20% Rat heart tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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