CF®488A Goat Anti-Human IgM (Mu Chain), 2mg/mL

Cat# 20347-50uL

Size : 50uL

Brand : Biotium

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Goat Anti-Human IgM (Mu Chain)

Goat anti-human IgM (mu chain) secondary antibody labeled with our superior CF® dyes.

Technology

CF® Dyes
Considerations for Immunofluorescence Staining
Secondary Antibody Product Listings

Tech Tips

Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host

Product Description

This is a goat anti-human IgM (mu chain) secondary antibody labeled with our bright and photostable CF® dyes. The antibodies react specifically with IgM heavy chains (mu chains) and not with immunoglobulin light chains. To minimize cross reactivity, the antibodies are cross-adsorbed against  human IgG prior to conjugation.

  • Cross-adsorbed for specific staining with minimal background
  • Available in 4 bright and photostable CF® dyes
  • Suitable for western, immunofluorescence, and immunohistology
Conjugation Ex/Em Size Catalog No. Dye Features
CF®488A 490/515 nm 50 uL (100 ug) 20347-50uL CF®488A Features
0.25 mL (500 ug) 20347-250uL
CF®594 593/614 nm 50 uL (100 ug) 20348-50uL CF®594 Features
0.25 mL (500 ug) 20348-250uL
CF®640R 642/662 nm 50 uL (100 ug) 20349-50uL CF®640R Features
0.25 mL (500 ug) 20349-250uL
CF®647 650/665 nm 50 uL (100 ug) 20346-50uL CF®647 Features
0.25 mL (500 ug) 20346-250uL

View our full selection of bright and specific Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.