Reagents and instruments for immunology, cell biology and molecular biology.

CD95 (Fas) (Biotin)

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Cat# C2442-21A-Biotin-100ul

Size : 100ul

Brand : US Biological

C2442-21A-Biotin Rabbit Anti-CD95 (Fas) (Biotin)

Clone Type

Polyclonal

Host

mouse

Source

human

Isotype

IgG1

Grade

Affinity Purified

Applications

Crossreactivity

Hu Mk

Shipping Temp

Blue Ice

Storage Temp

-20°C

Applications: |Suitable for use in Flow Cytometry (Not Tested) and Immunoprecipitation. Not suitable for use in Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Flow Cytometry (Not Tested): 1:50-1:100|Immunoprecipitation: 20ug/ml. 10ul labels 10e6 cells in 100ul.|Optimal dilutions to be determined by the researcher.||Hybridoma: |NS1 myeloma cells with spleen cells from Balb/c mice. ||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: 3H1775 (LOB 3/17)|Host: mouse|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.|Purity: Purified by Protein A affinity chromatography.|Immunogen: Fusion protein comprising extracellular domain of human Fas linked to human Fc.|Specificity: Recognizes the human CD95 cell surface antigen, a 40-50kD glycoprotein expressed by activated T and B cells, NK cells and thymocytes. Species Crossreactivity: Rhesus monkey ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Fusion protein comprising extracellular domain of human Fas linked to human Fc.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

Purity

Purified by Protein A affinity chromatography.

Specificity

Recognizes the human CD95 cell surface antigen, a 40-50kD glycoprotein expressed by activated T and B cells, NK cells and thymocytes. Species Crossreactivity: Rhesus monkey

References

1. Mesdaghi, M. et al. (2010) Int Arch Allergy Immunol. 153: 234-38. 2. Ximeri, M. et al.. (2010) Haematologica. 95: 406-14. 3. Aref, S. et al. (2004) Hematol. J. 5: 434-9. 4. Welsh, J.P. et al. (2004) Hematol J. 5: 39-46. 5. Wethkamp, N. et al. (2011) J Biol Chem. 286: 19576-88. 6. Chen, J.Y. et al. (2003) Nephrol Dial Transplant. 18: 1741-7. 7. Papadaki, H.A. et al. (2002) Blood. 99: 1610-9. 8. Mavroudi, I. et al. (2011) J Leukoc Biol. 89: 771-83. 9. Pyrovolaki, K. et al. (2009) Arthritis Rheum. 60: 543-52. 10. Boula, A. et al. (2006) Clin Cancer Res. 12: 3099-108. 11. Papadaki, H.A. et al. (2005) Bone Marrow Transplant. 36: 1053-63.