Cd8a rat monoclonal antibody, clone YTS105.18, Low Endotoxin
Specifications
Product Data
Clone Name
YTS105.18
Application
FC, IHC
Recommended Dilution
Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells. Immunohistochemistry on Frozen Sections.
Reactivity
Mouse
Antibody Host
Rat
Isotype
IgG2a
Clonality
Monoclonal
Immunogen
Mouse spleen cells. Spleen cells from an immunised DA rat were fused with cells of the rat Y3/Ag1.2.3. myeloma cell line.
Specificity
SM024LE recognises a non polymorphic epitope on the mouse CD8 alpha chain. Antibodies produced by clone YTS105.18 have been reported to block MHC I dependent T-cell responses in vitro and in vivo, and induce transplantation tolerance in combination with CD4 antibodies.
CD8 T cell surface antigen is heterodimer of an alpha and a beta chain linked by two disulfide bonds. It belongs type I membrane protein. Selectively expressing of CD8 on a subset of T cells leads to CD8 T cell development. Through identifying cytotoxic/suppressor T-cells that interact with MHC class I bearing targets, CD8 is thought to play a role in the process of T-cell mediated killing. Veillette et al (1988) found the CD8 is associated with the internal membrane tyrosine-protein kinase p56lck.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
