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- Specifications
Product Description
Mouse monoclonal antibody raised against native CD47.
Immunogen
Native purified CD47 from African green monkey COS-7 cells.
Host
Mouse
Theoretical MW (kDa)
50-55
Reactivity
African green monkey, Human, Monkey, Non-Human Primates, Pig
Specificity
This antibody reacts with CD47 (Integrin Associated Protein), a 50-55 KDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines.
Form
Liquid
Conjugation
FITC
Isotype
IgM
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Flow Cytometry
- Publication Reference
- CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model.
Orazizadeh M, Lee HS, Groenendijk B, Sadler SJ, Wright MO, Lindberg FP, Salter DM.
Arthritis Research & Therapy 2008 Jan; 10(1):R4.
Application：Func, IHC, WB, Human, Human articular cartilage, normal and osteoarthritic chondrocytes.
- Blockade of thrombospondin-1-CD47 interactions prevents necrosis of full thickness skin grafts.
Isenberg JS, Pappan LK, Romeo MJ, Abu-Asab M, Tsokos M, Wink DA, Frazier WA, Roberts DD.
Annals of Surgery 2008 Jan; 247(1):180.
Application：WB-Ce, Human, HUVECs.
- Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages.
Ide K, Wang H, Tahara H, Liu J, Wang X, Asahara T, Sykes M, Yang YG, Ohdan H.
PNAS 2007 Mar; 104(12):5062.
- CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model.