CD277 antibody
Cat# orb666153-50ul
Size : 50ul
Brand : Biorbyt
Item 1 of 2
Item 1 of 2
CD277 antibody
Catalog Number: orb666153
| Catalog Number | orb666153 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to CD277 |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IH, WB |
| Reactivity | Human, Mouse, Rat |
| Immunogen | Recombinant fusion protein of human CD277. The exact sequence is proprietary. |
| Dilution range | WB: 1-500-2000, IF: 1-50-200 |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Conjugation | Unconjugated |
| Target | BTN3A1 |
| Entrez | 11119 |
| UniProt ID | O00481 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Alternative names | Anti-BTF5 antibody, anti-Butyrophilin subfamily 3 |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of CD277 expression in HL60 (A), U937 (B) whole cell lysates. (Predicted band size: 39; 41; 52; 57 kD; Observed band size: 56 kD)
Immunofluorescent analysis of CD277 staining in mouse lung tissues. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.