Reagents and instruments for immunology, cell biology and molecular biology.

CD134 Ligand CD8a, Fusion Protein (Biotin)

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Cat# C2515-40G-25ug

Size : 25ug

Brand : US Biological

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C2515-40G CD134 Ligand CD8a, Fusion Protein (Biotin)

Clone Type

Polyclonal

Grade

Affinity Purified

Applications

E FC

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

Human CD134L (OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L is involved with costimulatory activity between B cells and T cells (1). CD134L-muCD8 fusion protein binds to CD134 positive cells in Flow cytometry||Functional Application: CD134L-muCD8 fusion protein binds to CD134 positive cells in Flow cytometry.||Molecular Structure: A soluble molecule consisting of the extracellular domain (132 aa) of human CD134L fused to the extracellular domain (167aa) of murine CD8 alpha.||Transfectant Cell Line: CHO||Applications: |Suitable for use in ELISA, Flow cytometry. Other applications not tested.||Recommended Dilution|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.|

Applications

Source: CHO cells|Purity: Purified by affinity chromatography.|Concentration: ~0.5mg/ml|Form: Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3.

Purity

Purified by affinity chromatography.

References

1) W. R. Godfrey, et al, (1994) J Exp Med 180: 757-763. |2) L.M. Higgins, et al, (1999) J Immunol 162: 486-493. |3) A.D. Weinberg, et al, (1999) J Immunol 162: 1818-1826. |4) V.Y. Taraban, et al, (2002) Eur J. Immunol. 32: 3617-3627. |5) V. Malmstrom, et al, (2001) J Immunol 166: 6972. |6) C. Nohara, et al, (2001) J Immunol 166: 2108-2115. |7) X. Yuan, et al, (2003) J Immunol 170: 2949-2955. |8) L. Tian, et al, (2002) Transplantation 74(1): 133-138.