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> Catenin, beta (CTNNB1) (rCTNNB1/2173), 1mg/mL

Catenin, beta (CTNNB1) (rCTNNB1/2173), 1mg/mL

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Cat# BNUM2173-50

Size : 50uL

Brand : Biotium

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Formalin-fixed paraffin-embedded human Breast Cancer stained with Beta-Catenin Mouse Recombinant Monoclonal Ab (rCTNNB1/2173).
SDS-PAGE Analysis Purified Beta-Catenin Mouse Recombinant Monoclonal Ab (rCTNNB1/2173). Confirmation of Purity and Integrity of Antibody.
Confocal Immunofluorescence image of HeLa cells using Beta-Catenin Mouse Recombinant Monoclonal Ab (rCTNNB1/2173).Green (CF488) and Reddot is used to label the nuclei.
Analysis of Protein Array containing >19000 full-length human proteins using Beta-Catenin Mouse Recombinant Monoclonal Antibody (rCTNNB1/2173) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target if the MAb has an S-score of at least 2.5. For example if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14 then the S-score for the binding of that MAb to protein X is equal to 29.
Antibody number

#2173
Antibody reactivity (target)

Beta-catenin, Catenin, Beta
Antibody type

Primary
Host species

Mouse
Clonality

Recombinant Monoclonal
Clone

rCTNNB1/2173
Isotype

IgG1, kappa
Molecular weight

92 kDa
Synonyms

Beta-catenin; Catenin beta-1; Catenin (Cadherin associated protein), beta 1; CTNNB
Human gene symbol

CTNNB1
Entrez gene ID

1499
SwissProt

P35222
Unigene

476018
Immunogen

Recombinant full-length human β-catenin protein
Verified antibody applications

IF (verified), IHC (FFPE) (verified), WB (verified)
Antibody target cellular localization

Plasma membrane, Nucleus
Species reactivity

Human, Mouse, Rat
Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes, Optimal dilution for a specific application should be determined by user
Positive control

HeLa or MCF-7 cells. Breast carcinoma
Shipping condition

Room temperature
Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status

For research use only (RUO)
Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Validated in protein array

Monospecific
Antibody research areas

Cancer, Cell adhesion, Developmental biology, Signal transduction
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.
Cell/tissue expression

Epithelial cells
Tumor expression

Breast cancer
Protocols

PI Protocols for Antibody Based Detection

SDS

SDS Primary Antibodies

Download Datasheet

Product Description

Beta-catenin associates with the cytoplasmic portion of E-cadherin. The catenin/cadherin complexes play an important role mediating cellular adhesion, including adherens junctions. Beta-catenin is involved in the Wnt signaling pathway as well as other signaling pathways, and is also found in complexes with many different proteins including the tumor suppressor protein APC. Defects in beta-catenin are associated with colorectal cancer, as well as many other cancer types. Beta-catenin is normally localized to the cell membrane, but can translocate to the nucleus in response to certain cell signaling.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the

CF® Dye Brochure

for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email

info@biotrend-usa.com

to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 2173, Anti-Beta-Catenin (rCTNNB1/2173) 

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405

CF®405S Features
BNC88 CF®488A 490/515 488 GFP, FITC

CF®488A Features
BNC68 CF®568 562/583 532, 561 RFP, TRITC

CF®568 Features
BNC94 CF®594 593/614 561 Texas Red®

CF®594 Features
BNC40 CF®640R 642/662 633-640 Cy®5

CF®640R Features
BNC47 CF®647 650/665 633-640 Cy®5

CF®647 Features
BNC74 CF®740 742/767 633-685 775/50

CF®740 Features
BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free		 N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.