Reagents and instruments for immunology, cell biology and molecular biology.
 
> Catalase (CAT) Activity Assay Kit

Catalase (CAT) Activity Assay Kit

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Cat# E-BC-K031-M-96T

Size : 96T

Brand : Elabscience

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Detection Principle The reaction that catalase (CAT) decomposes H2O2 can be quickly stopped by ammonium molybdate. The residual H2O2 reacts with ammonium molybdate to generate a yellowish complex. CAT activity can be calculated by production of the yellowish complex at 405 nm.
Synonyms CAT
Sample Type Serum,Plasma,Cell,Cell culture supernatant,Tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (400-410 nm, optimum wavelength: 405 nm)
Research Area Oxidative Stress
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 1.12 U/mL
Detection Range 1.12-150 U/mL
Precision inter-assay CV: 7.7% | intra-assay CV: 3.9%
Assay Time 40 min
Cat.No. Product Name Sizes
E-BC-K020-M Total Superoxide Dismutase (T-SOD) Activity Assay Kit (WST-1 Method) 500Assays , 96T , 48T
E-BC-K025-M Malondialdehyde (MDA) Colorimetric Assay Kit (TBA Method) 500Assays , 96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
293T supernatant 1
10% Rat heart tissue homogenization 50-100
10% Rat liver tissue homogenization 100-200
10% Rat spleen tissue homogenization 50-100
Mouse serum 1
10% Epipremnum aureum tissue homogenization 1-2
10% Rat lung tissue homogenization 50-100
10% Rat kidney tissue homogenization 50-100
10% Rat brain tissue homogenization 20-50

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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