Cat Trypsin, TRP ELISA Kit
Cat# E0077Cat-5X96wells
Size : 5X96wells
Brand : Bioassay Technology Laboratory
Product overview
Full product name
Trypsin
Code
E0077Cat
Assay type
Sandwich
Size
48T,96T
Sensitivity
0.28ng/ml
Detection range
0.5-200ng/ml
Sample type
Serum, plasma, cell culture supernates
Species
Cat
Storage
2-8ºC
Assay time
1h 30m
UniProt accession
MASS(Da)
1,825
GeneID
101093891
Synonyms
Fragment;MTG;Prss1;Prss2;TRP1;TRY2;TRY3;TRY4;Trypsin;
Gene names
Trypsin
Research area
Signal Transduction
Target protein
Trypsin
Components
Components | Quantity |
---|---|
Pre-coated ELISA plate | 12 * 8 well strips x1 |
Standard solution | 0.5ml x1 |
Standard diluent | 3ml x1 |
Streptavidin-HRP | 6ml x1 |
Stop solution | 6ml x1 |
Substrate solution A | 6ml x1 |
Substrate solution B | 6ml x1 |
Wash buffer concentrate (25x) | 20ml x1 |
Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0077Cat are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
120ng/ml | 2.349 | 2.354 | 2.295 |
2.359 | |||
60ng/ml | 1.471 | 1.478 | 1.419 |
1.484 | |||
30ng/ml | 0.759 | 0.764 | 0.705 |
0.769 | |||
15ng/ml | 0.554 | 0.546 | 0.487 |
0.539 | |||
7.5ng/ml | 0.285 | 0.283 | 0.224 |
0.282 | |||
0ng/ml | 0.068 | 0.059 | 0 |
0.049 |
Precision
We measured random samples of E0077Cat within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-Assay | 1 | 18 | 55.86 | 2.15 | 3.84 |
Intra-Assay | 2 | 18 | 13.75 | 0.82 | 5.99 |
Intra-Assay | 3 | 18 | 31.68 | 1.97 | 6.21 |
Protocols
Customer reviews and Q&As