Calpain 1/CAPN1 Antibody
Cat# orb251513-100ug
Size : 100ug
Brand : Biorbyt
Item 1 of 4
Item 1 of 4
Calpain 1/CAPN1 Antibody
Catalog Number: orb251513
| Catalog Number | orb251513 |
|---|---|
| Category | Antibodies |
| Description | Calpain 1/CAPN1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, ICC, IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555). Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 81890 MW |
| UniProt ID | P07384 |
| Sensitivity | < 5pg/ml |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Alternative names | Calpain-1 catalytic subunit;3.4.22.52;Calcium-acti |
| Note | For research use only |
| Application notes | WB: The detection limit for Calpain 1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of PC-3 cells using anti-Calpain 1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of Calpain 1 using anti-Calpain 1 antibody.Lane 1:human Jurkat cell; 2:human HeLa cell; 3:human K562 cell; 4:human PC-3 cell; 5:human T47D cell; 6:human A549 cell; 7:rat testis tissue; 8:mouse lung tissue.
IF analysis of Calpain 1 using anti-Calpain 1 antibody. Calpain 1 was detected in immunocytochemical section of U20S cells.
IHC analysis of Calpain 1 using anti-Calpain 1 antibody. Calpain 1 was detected in a paraffin-embedded section of Human Mammary Cancer tissue.