E-EL-0158-24T | Bovine/Sheep Cortisol ELISA Kit Biotrend USA

Product Summary

Sensitivity	0.96 ng/mL
Detection Range	1.56-100 ng/mL
Sample Volume	50 μL
Total Assay Time	2 h 30 min
Reacitivity	Bovine;Sheep
Specificity	This kit recognizes Bovine and Sheep Cortisol in samples.No significant cross-reactivity or interference between Bovine and Sheep Cortisol and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine and Sheep Cortisol. During the reaction, Bovine and Sheep Cortisol in the sample or standard competes with a fixed amount of Bovine and Sheep Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Bovine and Sheep Cortisol. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Bovine and Sheep Cortisol in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Background

Cortisol is the primary glucocorticoid hormone released by the body in response to stress, with chronic cortisol levels leading to various pathophysiologies.This hormone is regulated by the hypothalamic–pituitary–adrenal (HPA) axis, a complex neuroendocrinological system . Most cortisol in blood (90%) is bound to a carrier protein, cortisol binding globulin (CBG), with free cortisol only making up 5–10% of the total cortisol in circulation.Only free cortisol is biologically active, with bound cortisol being physiologically inactive. Cortisol controls a wide range of physiological processes, such as promoting gluconeogenesis, reducing inflammation, suppressing the immune system and modulating cognitive processes.These responses anticipate and assist the body’s adaptation to stressful conditions by providing energy for awakening, fuelling a “fight or flight” response, and diverting resources to deal with a stressor.

Research Area

Signal Transduction , Metabolism

Species

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All

Bovine

Bovine/Sheep

Canine

Human

Monkey

Porcine

Rabbit

Sheep

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Bovine/Sheep Cortisol ELISA Kit

Cat# E-EL-0158-24T

Brand : Elabscience

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