Blocking Buffer (2X) For Fluorescent Western Blotting
Cat# orb348639-50ml
Size : 50ml
Brand : Biorbyt
Blocking Buffer (2X) For Fluorescent Western Blotting
Catalog Number: orb348639
Western Blot Comparison of 1X and 2X orb348637 Buffer. Lane 1: reduced human NAG1 protein 0.1 µg. Lane 2: Prestained Molecular Weight Marker 5 µL. Lane 3: non-reduced human NAG1 protein 0.1 µg. Primary Antibody: rabbit anti-NAG1 biotin conjugated antibody (p/n orb345179) at 1 µg/mL overnight at 2-8°C. Secondary Antibody: goat anti-rabbit antibody HRP conjugated (p/n orb347654) at 1:70000 for 30 min at RT. Expect: ~14 kDa for NAG1.