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Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Birch pollen allergen recombinant monoclonal antibody, clone BV16 (Cat # RAB03100).
Binding curve of RAB03100 to ELISA Plate coated with BETVIA at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using RAB03100. For detection- a 1:4000 dilution of HRP-loat anti-ribody (Bio-Rad) was used. e at 450nm does not reach a plateau but falls slightly before increasing once more- as also observed for 3.0. This is suggestive of two binding sites on Bet v 1-A for which RAB03100 has differing affinities.
- Specifications
Product Description
Rabbit recombinant monoclonal antibody raised against Birch pollen allergen (BETVIA).
Antibody Species
Rabbit
Immunogen
Original antibody is raised against Birch pollen allergen, a major allergen of the European white birch.
Reactivity
Plants
Form
Liquid
Isotype
IgG kappa
Recommend Usage
Crystallography
ELISA
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing. - Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of Birch pollen allergen recombinant monoclonal antibody, clone BV16 (Cat # RAB03100).
Binding curve of RAB03100 to ELISA Plate coated with BETVIA at a concentration of 2 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using RAB03100. For detection- a 1:4000 dilution of HRP-loat anti-ribody (Bio-Rad) was used. e at 450nm does not reach a plateau but falls slightly before increasing once more- as also observed for 3.0. This is suggestive of two binding sites on Bet v 1-A for which RAB03100 has differing affinities.Crystallography