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> Biotin(Hyb8), Horseradish Peroxidase conjugate, 0.1mg/mL

Biotin(Hyb8), Horseradish Peroxidase conjugate, 0.1mg/mL

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Cat# BNCH0400-500

Size : 500uL

Brand : Biotium

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Biotin Monoclonal Mouse Antibody (Hyb8)

Product Attributes

Antibody number

#0400
Antibody reactivity (target)

Biotin
Antibody type

Primary, Tag Antibody
Host species

Mouse
Clonality

Monoclonal
Clone

Hyb8
Isotype

IgG1
Molecular weight

244Da
Synonyms

Vitamin B7 or Vitamin H
Human gene symbol

Not Applicable
Entrez gene ID

Not Applicable
SwissProt

Not Applicable
Unigene

Not Applicable
Immunogen

Biotinylated sheep immunoglobulin
Verified antibody applications

IHC (FFPE) (verified)
Species reactivity

All species
Antibody application notes

Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 0.5-1 ug/mL, No special pretreatment is required for the immunohistochemical staining of formalin-fixed paraffin-embedded tissues., Immunohistology formalin-fixed 0.5-1 ug/mL, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Western blotting
Positive control

Biotinylated proteins in solution or on tissues
Shipping condition

Room temperature
Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status

For research use only (RUO)
Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Technology

CF® Dyes
Considerations for Immunofluorescence Staining
Primary & Secondary Antibody Conjugates
Protocols
SDS
Supporting Documents
Download Datasheet

Product Description

This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the CF® Dye Brochure for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Stock status: Because Biotium offers a large number of antibody and conjugation options, primary antibody conjugates may be made to order. Typical lead times are up to one week for CF® dye and biotin conjugates, and up to 2-3 weeks for fluorescent protein and enzyme conjugates. Please email info@biotrend-usa.com to inquire about stock status and lead times before placing your order.

 Catalog number key for antibody number 0400, Anti-Biotin (Hyb8) 

Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405 CF®405S Features
BNC88 CF®488A 490/515 488 GFP, FITC CF®488A Features
BNC68 CF®568 562/583 532, 561 RFP, TRITC CF®568 Features
BNC94 CF®594 593/614 561 Texas Red® CF®594 Features
BNC40 CF®640R 642/662 633-640 Cy®5 CF®640R Features
BNC47 CF®647 650/665 633-640 Cy®5 CF®647 Features
BNC74 CF®740 742/767 633-685 775/50 CF®740 Features
BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free		 N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.

Note: Listed references are for this antibody clone sold by Biotium and other suppliers.