- More Files
Western Blot (Cell lysate)
Western blot analysis of BECN1 in HeLa whole cell lysate using BECN1 polyclonal antibody (Cat # PAB12473) at 1 : 10000.
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BECN1.
Immunogen
A synthetic peptide corresponding to amino acids 150-300 of human BECN1.
Host
Rabbit
Reactivity
Bovine, Chicken, Human, Mouse, Pig, Primates, Rat, Xenopus
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Immunocytochemistry (1:50)
Immunofluorescence (1:50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:400)
Western Blot (1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 0.1% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Cell lysate)
Western blot analysis of BECN1 in HeLa whole cell lysate using BECN1 polyclonal antibody (Cat # PAB12473) at 1 : 10000.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry
Immunocytochemistry
Immunofluorescence
- Gene Info — BECN1
Entrez GeneID
8678Protein Accession#
Q14457Gene Name
BECN1
Gene Alias
ATG6, VPS30, beclin1
Gene Description
beclin 1, autophagy related
Omim ID
604378Gene Ontology
HyperlinkGene Summary
O
Other Designations
ATG6 autophagy related 6 homolog|beclin 1|beclin 1 (coiled-coil, moesin-like BCL2 interacting protein)|beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)
- Interactomes
- Pathways
- Diseases
- Publication Reference
- Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy.
Pattingre S, Bauvy C, Carpentier S, Levade T, Levine B, Codogno P.
The Journal of Biological Chemistry 2009 Jan; 284(5):2719.
Application：IP, WB, Human, HeLa, MCF-7 cells.
- Consequences of the selective blockage of chaperone-mediated autophagy.
Massey AC, Kaushik S, Sovak G, Kiffin R, Cuervo AM.
PNAS 2006 Apr; 103(15):5805.
- Autophagy as a regulated pathway of cellular degradation.
Klionsky DJ, Emr SD.
Science 2000 Dec; 290(5497):1717.
Application：IHC, WB, Human, Cancers, Mammalican cells.
- Role of JNK1-dependent Bcl-2 phosphorylation in ceramide-induced macroautophagy.