ATP-polyamine-biotin [1800401-93-7]
Cat# HY-D0183-1mg
Size : 1mg
Brand : MedChemExpress
ATP-polyamine-biotin, the first cell-permeable ATP analogue, is an efficient kinase cosubstrate. ATP-polyamine-biotin promotes biotin labeling of kinase substrates in live cells.
For research use only. We do not sell to patients.
ATP-polyamine-biotin Chemical Structure
CAS No. : 1800401-93-7
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Description |
ATP-polyamine-biotin, the first cell-permeable ATP analogue, is an efficient kinase cosubstrate. ATP-polyamine-biotin promotes biotin labeling of kinase substrates in live cells[1]. |
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In Vitro |
ATP-polyamine-biotin (APB) is incubated with PKA kinase and full-length protein substrate, myelin basic protein (MBP). Biotinylation was observed only in presence of kinase. In addition, MBP biotinylation was lost in the absence of ATP-polyamine-biotin, in presence of the kinase inhibitor staursporine or upon incubation with acid due to cleavage of phosphoramidate bond. Biotinylated kemptide product is observed only in the presence of APB cosubstrate[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
945.85 |
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Formula |
C32H58N11O14P3S |
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CAS No. |
1800401-93-7 |
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Appearance |
Solid |
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Color |
White to off-white |
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SMILES |
O=C(CCCC[C@@H]1SC[C@]([C@]1(04)N2)(04)NC2=O)NCCCN(C)CCCCN(C)CCCNP(O)(OP(O)(OP(O)(OC[C@@H]3[C@@H](O)[C@@H](O)[C@H](N4C(N=CN=C5N)=C5N=C4)O3)=O)=O)=O |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
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Solvent & Solubility |
In Vitro:
H2O : 6 mg/mL (6.34 mM; Need ultrasonic and warming) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles. * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
For the following dissolution methods, please prepare the working solution directly.
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
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Purity & Documentation |
Purity: 98.06% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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H2O | 1 mM | 1.0573 mL | 5.2863 mL | 10.5725 mL | 26.4313 mL |
5 mM | 0.2115 mL | 1.0573 mL | 2.1145 mL | 5.2863 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
ATP-polyamine-biotin Related Classifications
- Others
- Others
- Biochemical Assay Reagents
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
ATP-polyamine-biotin1800401-93-7Biochemical Assay ReagentsATPanaloguesbiotinkinasesubstratescell-permeableAPBMBPInhibitorinhibitorinhibit