Reagents and instruments for immunology, cell biology and molecular biology.
 
> ATP Chemiluminescence Assay Kit

ATP Chemiluminescence Assay Kit

Print Print
Please log in

Cat# E-BC-F002-48T

Size : 48T

Brand : Elabscience


Detection principle

Under the catalyzation of luciferase, ATP react with luciferin and emits fluorescence, and the fluorescence intensity is proportional to the concentration of ATP within a certain range.

Performance characteristics

Synonyms ATP
Sample type Animal tissue, Cells
Sensitivity 0.003 μmol/L
Detection range 0.003-5 μmol/L
Detection method Fluorescence method
Assay type Quantitative
Assay time 15 min
Precision Average inter-assay CV: 6.500%Average intra-assay CV: 2.200%
Other instruments required Vortex mixer, Centrifuge
Storage -20℃
Valid period 12 months

Images

ATP level was significantly reduced in SB225002 cells. (*P<0.05, **P<0.01)

H Wang et al investigate the overexpression of CXCR2 on monocytes of traumatic brain injury and its effect. ATP level in cell supernatant was determined using ATP chemiluminescence assay kit (E-BC-F002).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.003-10 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Mouse heart tissue homogenate

1

10% Mouse kidney tissue homogenate

1

10% Mouse muscle tissue homogenate

1

10% Mouse liver tissue homogenate

1

10% Mouse brain tissue homogenate

1

10% Mouse lung tissue homogenate

1

Note: The diluent is reagent 1.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

  1. Adipocyte (2022) IF: 3.553
    Salvianolic acid A promotes mitochondrial biogenesis and mitochondrial function in 3T3-L1 adipocytes through regulation of the AMPK-PGC1α signalling pathway

    DOI: 10.1080/21623945.2022.2116790

    PMID: 36053001

    		 Sample: 3T3-L1 Adipocytes
  2. Journal of Neuroinflammation (2022) IF: 9.587
    Transient post-operative overexpression of CXCR2 on monocytes of traumatic brain injury patients drives monocyte chemotaxis toward cerebrospinal fluid and enhances monocyte-mediated immunogenic cell death of neurons in vitro

    DOI: 10.1186/s12974-022-02535-6

    PMID: 35768823

    		 Sample: Sh-Sy5Y Cell