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Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) with Tocilizumab. While the antibody does not recognize the human IgG (data not shown).Coating antigen: Tocilizumab, 1 ug/mL. Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) dilutions start from 1000 ng/mL. EC50 = 1.837 ng/mL.
Sandwich ELISA
Standard curve of Tocilizumab Sandwich ELISA. The Tocilizumab Sandwich ELISA assay is developed by using Anti-Tocilizumab monoclonal antibody, clone 14B10 (Cat # MAB23095) and Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) was labeled with Biotin. The sensitivity is less than 100 pg/mL.
Blocking
Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) blocks Tocilizumab binding with Human IL-6R (IL-6R, Human). Coating antigen: IL-6R, 0.5 ug/mL. Tocilizumab-biotin final concentration: 0.5 ug/mL. Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) dilutions start from 10 ug/mL. IC50 = 0.656 ug/mL.
- Specifications
Product Description
Mouse monoclonal antibody raised against Tocilizumab.
Target gene is IL-6 receptor.Immunogen
Tocilizumab.
Host
Mouse
Specificity
The product is specific for Tocilizumab. The antibody is recommended as a detection antibody in a pharmacokinetic (PK) bridging assay with capture antibody Anti-Tocilizumab monoclonal antibody, clone 14B10 (Cat # MAB23095).
Form
Lyophilized
Preparation Method
This antibody is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with Tocilizumab.
Purification
Protein A purification
Isotype
IgG2b
Recommend Usage
ELISA (ELISA detection: 0.01-0.1 ug/mL; ELISA blocking: 1-30 ug/mL) (Direct/Indirect/Inhibitory ELISA)
Sandwich ELISA
The optimal working dilution should be determined by the end user.Inhibitory IC50
0.656 ug/mL
Storage Buffer
Lyophilized from PBS, pH 7.4 (0.02% sodium azide).
Storage Instruction
Store at -20°C on dry atmospher. The lyophilized product remains stable up to 1 year at -20°C from date of receipt.
After reconstitution with deionized water (or equivalent) to a final concentration of 0.5 mg/mL, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Enzyme-linked Immunoabsorbent Assay
ELISA binding of Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) with Tocilizumab. While the antibody does not recognize the human IgG (data not shown).Coating antigen: Tocilizumab, 1 ug/mL. Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) dilutions start from 1000 ng/mL. EC50 = 1.837 ng/mL.Sandwich ELISA
Standard curve of Tocilizumab Sandwich ELISA. The Tocilizumab Sandwich ELISA assay is developed by using Anti-Tocilizumab monoclonal antibody, clone 14B10 (Cat # MAB23095) and Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) as the capture and detection antibodies, respectively. In this ELISA assay, Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) was labeled with Biotin. The sensitivity is less than 100 pg/mL.Blocking
Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) blocks Tocilizumab binding with Human IL-6R (IL-6R, Human). Coating antigen: IL-6R, 0.5 ug/mL. Tocilizumab-biotin final concentration: 0.5 ug/mL. Anti-Tocilizumab monoclonal antibody, clone 6C10 (Cat # MAB23096) dilutions start from 10 ug/mL. IC50 = 0.656 ug/mL. - Gene Info — IL6R
Entrez GeneID
3570Gene Name
IL6R
Gene Alias
CD126, IL-6R-1, IL-6R-alpha, IL6RA, MGC104991
Gene Description
interleukin 6 receptor
Omim ID
147880Gene Ontology
HyperlinkGene Summary
Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. The protein encoded by this gene is a subunit of the receptor complex for IL6. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
CD126 antigen|OTTHUMP00000034320|interleukin 6 receptor alpha subunit
- Interactomes
- Pathways
- Diseases