Anti-SREBF1 antibody (250-330 aa)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	SREBF1
Gene ID:	6720
Uniprot ID:	SRBP1_HUMAN
Immunogen Region:	250-330 aa
Specificity:	This antibody detects endogenous levels of SREBP-1
Immunogen:	Synthesized peptide derived from the human SREBP-1 at the amino acid range 250-330

Post Translational Modifications	Sterol regulatory element-binding protein 1: Processed in the Golgi apparatus, releasing the protein from the membrane. At low cholesterol the SCAP-SREBP complex is recruited into COPII vesicles for export from the endoplasmic reticulum. In the Golgi, complex SREBPs are cleaved sequentially by site-1 (MBTPS1, S1P) and site-2 (MBTPS2, S2P) protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain, releasing the transcription factor from the Golgi membrane. Phosphorylated by AMPK, leading to suppress protein processing and nuclear translocation, and repress target gene expression. Phosphorylation at Ser-402 by SIK1 represses activity possibly by inhibiting DNA-binding. Processed sterol regulatory element-binding protein 1: Ubiquitinated.the nuclear form has a rapid turnover and is rapidly ubiquitinated and degraded by the proteasome in the nucleus.
Function	Sterol regulatory element-binding protein 1: Precursor of the transcription factor form (Processed sterol regulatory element-binding protein 1), which is embedded in the endoplasmic reticulum membrane. Low sterol concentrations promote processing of this form, releasing the transcription factor form that translocates into the nucleus and activates transcription of genes involved in cholesterol biosynthesis and lipid homeostasis. Processed sterol regulatory element-binding protein 1: Key transcription factor that regulates expression of genes involved in cholesterol biosynthesis and lipid homeostasis. Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3'). Regulates the promoters of genes involved in cholesterol biosynthesis and the LDL receptor (LDLR) pathway of sterol regulation. Isoform SREBP-1A: Isoform expressed only in select tissues, which has higher transcriptional activity compared to SREBP-1C. Able to stimulate both lipogenic and cholesterogenic gene expression. Has a role in the nutritional regulation of fatty acids and triglycerides in lipogenic organs such as the liver. Required for innate immune response in macrophages by regulating lipid metabolism. Isoform SREBP-1C: Predominant isoform expressed in most tissues, which has weaker transcriptional activity compared to isoform SREBP-1A. Primarily controls expression of lipogenic gene. Strongly activates global lipid synthesis in rapidly growing cells. Isoform SREBP-1aDelta: The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters. Isoform SREBP-1cDelta: The absence of Golgi proteolytic processing requirement makes this isoform constitutively active in transactivation of lipogenic gene promoters.
Protein Name	Sterol Regulatory Element-Binding Protein 1 Srebp-1 Class D Basic Helix-Loop-Helix Protein 1 Bhlhd1 Sterol Regulatory Element-Binding Transcription Factor 1 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 1 Transcription Factor Srebf1
Database Links	Reactome: R-HSA-1368082 Reactome: R-HSA-1655829 Reactome: R-HSA-191273 Reactome: R-HSA-1989781 Reactome: R-HSA-2426168 Reactome: R-HSA-381340 P36956-1 Reactome: R-HSA-9029558 P36956-3 Reactome: R-HSA-9615017
Cellular Localisation	Sterol Regulatory Element-Binding Protein 1: Endoplasmic Reticulum Membrane Multi-Pass Membrane Protein Golgi Apparatus Membrane Cytoplasmic Vesicle Copii-Coated Vesicle Membrane At High Sterol Concentrations The Scap-Srebp Is Retained In The Endoplasmic Reticulum Low Sterol Concentrations Promote Recruitment Into Copii-Coated Vesicles And Transport Of The Scap-Srebp To The Golgi Where It Is Processed Processed Sterol Regulatory Element-Binding Protein 1: Nucleus Isoform Srebp-1adelta: Nucleus Isoform Srebp-1cdelta: Nucleus
Alternative Antibody Names	Anti-Sterol Regulatory Element-Binding Protein 1 antibody Anti-Srebp-1 antibody Anti-Class D Basic Helix-Loop-Helix Protein 1 antibody Anti-Bhlhd1 antibody Anti-Sterol Regulatory Element-Binding Transcription Factor 1 Cleaved Into - Processed Sterol Regulatory Element-Binding Protein 1 antibody Anti-Transcription Factor Srebf1 antibody Anti-SREBF1 antibody Anti-BHLHD1 antibody Anti-SREBP1 antibody

Information sourced from Uniprot.org

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