Reagents and instruments for immunology, cell biology and molecular biology.

Anti-SEC23A antibody (300-400) (Clone: S2MR)

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Cat# NB-22-48761-100

Size : 100uL

Brand : Neo Biotech

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Western blot analysis of extracts of HepG2 cells, using Sec23AA rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunofluorescence analysis of NIH/3T3 cells using Sec23AA Rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry of paraffin-embedded rat ovary using Sec23AA rabbit monoclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Western blot analysis of extracts of Hep G2 cells, using Sec23AA Rabbit monoclonal antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human appendix using Sec23AA rabbit monoclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using Sec23AA rabbit monoclonal antibody at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
Immunofluorescence analysis of NIH-3T3 cells using Sec23AA rabbit monoclonal antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

General Info

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-SEC23 (300-400) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	S2MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Information

Gene Symbol:	SEC23A
Gene ID:	10484
Uniprot ID:	SC23A_HUMAN
Immunogen Region:	300-400
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 300-400 of human Sec23AA (Q15436).
Immunogen Sequence:	MVVGDELKTPIRSWHDIDKD NAKYVKKGTKHFEALANRAA TTGHVIDIYACALDQTGLLE MKCCPNLTGGYMVMGDSFNT SLFKQTFQRVFTKDMHGQFK M

Description

Tissue Specificity	Ubiquitously expressed.
Function	Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex. Required for the translocation of insulin-induced glucose transporter SLC2A4/GLUT4 to the cell membrane.
Protein Name	Protein Transport Protein Sec23a Hsec23a Sec23-Related Protein A
Database Links	Reactome: R-HSA-1655829 Reactome: R-HSA-204005 Reactome: R-HSA-2132295 Reactome: R-HSA-5694530 Reactome: R-HSA-9705671 Reactome: R-HSA-983170
Cellular Localisation	Cytoplasmic Vesicle Copii-Coated Vesicle Membrane Peripheral Membrane Protein Cytoplasmic Side Endoplasmic Reticulum Membrane Cytoplasm Cytosol Enriched At Endoplasmic Reticulum Exit Sites Also Known As Transitional Endoplasmic Reticulum (Ter)
Alternative Antibody Names	Anti-Protein Transport Protein Sec23a antibody Anti-Hsec23a antibody Anti-Sec23-Related Protein A antibody Anti-SEC23A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance