Reagents and instruments for immunology, cell biology and molecular biology.

Anti-IgG, Lambda (FITC) Polyclonal Antibody

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Cat# I1904-44A-1mg

Size : 1mg

Brand : US Biological

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I1904-44A IgG, Lambda (FITC)

Clone Type

Polyclonal

Host

goat

Source

human

Isotype

IgG

Grade

Affinity Purified

Applications

FLISA IF WB

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

4°C (-20°C Glycerol)

Applications:|Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays. Other applications not tested.||Recommended Dilutions: |Optimal dilutions to be determined by the researcher.||Absorption Wavelength: 495nm||Emission Wavelength: 528nm||Fluorochrome/Protein Ratio:|2.7 moles FITC per mole of Goat IgG||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

Applications

Product Type: Pab|Isotype: IgG|Host: goat|Source: human|Concentration: ~1mg/ml (UV280)|Form: Supplied as a lyophilized powder from PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.|Purity: Purified by immunoaffinity chromatography using human antigens coupled to agarose beads followed by solid phase adsorption.|Immunogen: Human lambda free chain.|Specificity: Recognizes human IgG, Lambda. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Human lambda free chain.

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.

Purity

Purified by immunoaffinity chromatography using human antigens coupled to agarose beads followed by solid phase adsorption.

Specificity

Recognizes human IgG, Lambda. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other human heavy or light chain isotypes.