Anti-Heat Shock Protein 27 (Hsp27) Monoclonal Antibody
Cat# FAA693Hu04-100
Size : 100tests
Brand : USCN
Anti-Heat Shock Protein 27 (Hsp27) Monoclonal Antibody
HSPB1; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock 27kDa Protein 1; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;
- Product No.FAA693Hu04
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Potency (Clone Number)D3
- Ig Isotype IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFlow cytometry.
- Download n/a
- UOM 25test50test 100test 200test 500test
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Packages (Simulation) -
Packages (Simulation)
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Human MCF7 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 10µg/ml mouse Anti-human Hsp27 Monoclonal Antibody (Catalog FAA693Hu04, red histogram) or Isotype control antibody (Catalog IS067-Mu01, green histogram), followed by 1µg/ml FITC-conjugated Anti-mouse IgG Secondary Antibody (Catalog SAA544Mu18).
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ISO9001: 2008, ISO13485: 2003 Registered
USAGE of the Anti-Heat Shock Protein 27 (Hsp27) Monoclonal Antibody
Western blotting: 0.2-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:10μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE of the Anti-Heat Shock Protein 27 (Hsp27) Monoclonal Antibody
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.