General Info
Host: | Rabbit |
Applications: | ELISA/IHC/IP/WB |
Reactivity: | Human/Monkey/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Rabbit polyclonal antibody anti-CD4 (437-458) is suitable for use in ELISA, Immunohistochemistry, Immunoprecipitation and Western Blot research applications. |
Clonality: | Polyclonal |
Conjugation: | Biotin |
Isotype: | IgG |
Purification: | Affinity Purified |
Concentration: | 0.55-0.75 µg/µl |
Dilution Range: | WB: 1:500 DB: 1:10, 000 ELISA: 1:10, 000 IP: 1:150 IHC: 1:50-1:150 |
Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
Information
Description
Tissue Specificity | Highly expressed in T-helper cells. The presence of CD4 is a hallmark of T-helper cells which are specialized in the activation and growth of cytotoxic T-cells, regulation of B cells, or activation of phagocytes. CD4 is also present in other immune cells such as macrophages, dendritic cells or NK cells. |
Post Translational Modifications | Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Phosphorylated by PKC.phosphorylation at Ser-433 plays an important role for CD4 internalization. |
Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. (Microbial infection) Primary receptor for human immunodeficiency virus-1 (HIV-1). Down-regulated by HIV-1 Vpu. Acts as a receptor for Human Herpes virus 7/HHV-7. |
Protein Name | T-Cell Surface Glycoprotein Cd4 T-Cell Surface Antigen T4/Leu-3 Cd Antigen Cd4 |
Database Links | Reactome: R-HSA-1462054 Reactome: R-HSA-167590 Reactome: R-HSA-173107 Reactome: R-HSA-180534 Reactome: R-HSA-202424 Reactome: R-HSA-202427 Reactome: R-HSA-202430 Reactome: R-HSA-202433 Reactome: R-HSA-389948 Reactome: R-HSA-449836 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 |
Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Localizes To Lipid Rafts Removed From Plasma Membrane By Hiv-1 Nef Protein That Increases Clathrin-Dependent Endocytosis Of This Antigen To Target It To Lysosomal Degradation Cell Surface Expression Is Also Down-Modulated By Hiv-1 Envelope Polyprotein Gp160 That Interacts With And Sequesters Cd4 In The Endoplasmic Reticulum |
Alternative Antibody Names | Anti-T-Cell Surface Glycoprotein Cd4 antibody Anti-T-Cell Surface Antigen T4/Leu-3 antibody Anti-Cd Antigen Cd4 antibody Anti-CD4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance