Reagents and instruments for immunology, cell biology and molecular biology.

Anti-CD21 (CR2) (Biotin) Monoclonal Antibody

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Cat# 214612-Biotin-100ul

Size : 100ul

Brand : US Biological

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214612-Biotin CD21 (CR2) (Biotin)

Clone Type

Polyclonal

Host

mouse

Source

bovine

Isotype

IgG1

Grade

Affinity Purified

Applications

FC IF IHC IP

Crossreactivity

Bo Gt Sh

Shipping Temp

Blue Ice

Storage Temp

-20°C

Bovine CD21 cell surface, a145kD single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2. In cattle CD21 expression is restricted to B lymphocytes (Naessens et al. 1990). CD21 may be expressed on B cells as either a long or a short form (Pringle et al. 2012)||Applications: |Suitable for use in Immunofluorescence, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Optimal dilutions to be determined by the researcher.||Hybridoma: |NS1 myeloma cells with spleen cells from Balb/c mice. ||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: CC21|Host: mouse|Source: bovine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Specificity: Recognizes bovine CD21. Species Crossreactivity: goat, sheep, red deer and mule deer||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

Purity

Purified by Protein G affinity chromatography from tissue culture supernatant.

Specificity

Recognizes bovine CD21. Species Crossreactivity: goat, sheep, red deer and mule deer

References

1. Howard, C.J. et al. (1991) Summary of workshop findings for leukocyte antigens of cattle. Vet. Immunol. Immunopathol. 27: 21-27. 2. Naessens, J. et al. (1990) Characterization of a bovine leucocyte differentiation antigen of 145,000 Mw restricted to B lymphocytes. Immunology 69: 525-530. 3. Sopp, P. & Howard, C.J. (2001) IFN gamma and IL-4 production by CD4, CD8, and WC1 gamma-delta TCR+ cells from cattle lymph nodes and blood. Vet. Immunol. Immunopathol. 81: 85-96. 4. Lwin, S. et al. (2009) Immune cell types involved in early uptake and transport of recombinant mouse prion protein in Peyer's patches of calves. Cell Tissue Res. 338: 343-54. 5. Breugelmans, S. et al. (2011) Immunoassay of lymphocyte subsets in ovine palatine tonsils. Acta Histochem. 113: 416-22. 6. Halliday, S. et al. (2005) Expression of PrPC on cellular components of sheep blood. J. Gen. Virol. 86: 1571-1579. 7. Brackenbury, L.S. et al. (2005) Identification of a cell population that produces alpha/beta interferon in vitro and in vivo in response to noncytopathic bovine viral diarrhea virus. J Virol. 79: 7738-44. 8. Breugelmans, S. et al. (2011) Differences between the ovine tonsils based on an immunohistochemical quantification of the lymphocyte subpopulations. Comp Immunol Microbiol Infect Dis. 34: 217-25. 9. Richt, J.A. et al. (2007) Production of cattle lacking prion protein. Nat Biotechnol. 25: 132-8. 10. Brujeni, G.N. et al. (2010) Bovine immunodeficiency virus and bovine leukemia virus and their mixed infection in Iranian Holstein cattle. J Infect Dev Ctries. 4: 576-9. 11. Kiku, Y. et al. (2010) Decrease in bovine CD14 positive cells in colostrum is associated with the incidence of mastitis after calving. Vet Res Commun. 34: 197-203. 12. Pilla, R. et al. (2012) Long-term study of MRSA ST1, t127 mastitis in a dairy cow. Vet Rec. Mar 1. 13. Chattha, K.S. et al. (2010) Immunohistochemical investigation of cells expressing CD21, membrane IgM, CD32 and a follicular dendritic cell marker in the lymphoid tissues of neonatal calves. Vet Immunol Immunopathol. 137: 284-90. 14. Weiss, D.J. et al. (2006) Mucosal immune response in cattle with subclinical Johne's disease. Vet Pathol. 43: 127-35. 15. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma. Vet Immunol Immunopathol. 146: 1-7. 16. Sigurdson, C.J. et al. (2002) PrP(CWD) lymphoid cell targets in early and advanced chronic wasting disease of mule deer. J Gen Virol. 83: 2617-28. 17. Dagleish, M.P.et al. (2012) Immunophenotype of cells within cervine rectoanal mucosa-associated lymphoid tissue and mesenteric lymph nodes. J Comp Pathol. 146: 365-71. 18. Edwards, J.C. et al. (2010) PrP(Sc) is associated with B cells in the blood of scrapie-infected sheep. Virology. 405: 110-9. 19. Brodzki, P. et al. (2014) Phenotyping of leukocytes and granulocyte and monocyte phagocytic activity in the peripheral blood and uterus of cows with endometritis. Theriogenology 28 April