Animal TYR ELISA Kit
Cat# orb780919-48
Size : 48Test
Brand : Biorbyt
Catalog Number | orb780919 |
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Category | Assays and Kits |
Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosinase(TYR). |
Tested applications | ELISA |
Reactivity | Bovine |
Range | 7.82-500 ng/mL |
Concentration | 500 ng/mL |
UniProt ID | Q8MIU0 |
Sensitivity | 3.1 ng/mL |
Protein Length | 3.5h |
Alternative names | OCAIA ELISA Kit, OCA1A ELISA Kit, OCA1 ELISA Kit, |
Note | For research use only |
Application notes | Standard: 500 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle TYR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TYR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle TYR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TYR in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | Serum, plasma and other biological fluids. |
Expiration Date | Please enquire. |