Animal GAL8 ELISA Kit
Cat# orb779793-48
Size : 48Test
Brand : Biorbyt
| Catalog Number | orb779793 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 8(GAL8). |
| Tested applications | ELISA |
| Reactivity | Bovine |
| Range | 0.32-20 ng/mL |
| Concentration | 20 ng/mL |
| UniProt ID | Q2YDD7 |
| Sensitivity | 0.118 ng/mL |
| Protein Length | 3.5h |
| Alternative names | LGALS8 ELISA Kit, PCTA1 ELISA Kit, Po66-CBP ELISA |
| Note | For research use only |
| Application notes | Standard: 20 ng/mL. Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle GAL8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle GAL8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle GAL8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle GAL8 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Expiration Date | Please enquire. |