Amylin, Rat, Control Peptide (DAP, Diabetes-Associated Peptide, IAP, Islet Amyloid Polypeptide, Insulinoma Amyloid Peptide, IAPP)

Control peptide for A2275-53P.||The calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1, and CGRP2) and adrenomedullin (ADM). Amylin is a 37aa peptide produced in the pancreatic beta-cell secretory granules and is co-released with insulin. Amylin also has CGRP-like effects on bone metabolism. Amylin has specific binding sites in the CNS and it may regulate gastric emptying and influence carbohydrate metabolism. The calcitonin family peptides probably act through G-protein coupled membrane receptors. Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461aa; rat/mouse 463aa) was identified. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed.||Source:|Synthetic peptide corresponding to 13aa from the ~mid-region of mature of Amylin 18-30aa of rat AMYL/AML (accession #P12969).||Molecular Weight:|~3kD||Applications:|Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested.||Recommended Dilution:|ELISA: 5-10ug control peptide per 1 g of aff pure IgG or 1ul antiserum|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.||