AM4-65
Cat# 70039
Size : 1MG
Brand : Biotium
AM4-65
AM4-65 is a fixable red fluorescent nerve terminal probe. The dye is spectrally identical to SynaptoRed™ C2 (equivalent to FM®4-64), but it has an additional amine group at the hydrophilic end of the molecule, making the dye fixable with formaldehyde or gluteraldehyde.
Technology
Membrane Potential & Nerve Terminal Dyes
Neuronal Probes and Tools
Protocols
SDS
Supporting Documents
Product Description
AM4-65 is a fixable red fluorescent nerve terminal probe. The dye is spectrally identical to SynaptoRed™ C2 (equivalent to FM®4-64), but it has an additional amine group at the hydrophilic end of the molecule, making the dye fixable with formaldehyde or gluteraldehyde. The long emission wavelength makes the dye suitable to be used with GFP in two-color imaging.
- λEx/λEm (membranes) = ~510/750 nm*
- Dark purple solid soluble in water
- MW: 844.85
* Excitation/emission settings of 515/640 nm have been used for detection of yeast vacuole staining with SynaptoRed™ C2 (FM®4-64), and should be applicable to AM4-65.
Nerve Terminal Dyes and Kits
| Product | Catalog No. | Features |
|---|---|---|
| SynaptoGreen™ Dyes (Ex/Em ~480/660 nm in membranes) | 70020... 70053 | View full list of SynaptoGreen™ Dyes |
| SynaptoRed Dyes™ (Ex/Em ~510/750 nm in membranes) | 70021... 70050 | View full list of SynaptoRed™ Dyes |
| Background Reducers | ||
| ADVASEP-7 | 70029 | Sulfonated cyclodextrin that aids in removal of free dye during washes |
| SCAS | 70037 | Quenches extracellular fluorescences with fewer wash steps than ADVASEP-7 |
| Sulforhodamine 101 | 80101 | Red fluorescent dye that quenches extracellular fluorescence of SynaptoGreen™ dyes |
| Nerve Terminal Staining Kits | ||
| Nerve Terminal Staining Kit I | 70030 | Includes SynaptoGreen™ C4 and ADVASEP-7 |
| Nerve Terminal Staining Kit II (A) | 70031 | Includes AM1-43 and ADVASEP-7 |
| Nerve Terminal Staining Kit II (B) | 70031-1 | Includes AM1-43 and SCAS |
| Nerve Terminal Staining Kit III | 70032 | Includes SynaptoGreen™ C4 and Sulforhodamine 101 |
| Nerve Terminal Staining Kit V | 70034 | Includes SynaptoRed™ C2 and ADVASEP-7 |
**The positively-charged end of SynaptoRed C2M is a trimethylammonium group.
FM is a registered trademark of Thermo Fisher Scientific.