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> Alpha-Bungarotoxin, CF®680R

Alpha-Bungarotoxin, CF®680R

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Cat# 00003

Size : 500UG

Brand : Biotium


α-Bungarotoxin, CF® Dye and Other Conjugates

Conjugates of α-Bungarotoxin labeled with a selection of our CF® Dyes and other labels. Labeled α-bungarotoxin conjugates can be used for staining nicotinic acetylcholine receptors at neuromuscular junctions in tissue sections.

α-Bungarotoxin, CF® Dye and Other Conjugates

Product Attributes

For live or fixed cells

For fixed cells, For live/intact cells
Cell permeability

Membrane impermeant
Fixation options

Fix before staining (formaldehyde), Fix before staining (methanol)
Assay type/options

Tissue staining
Toxin

Alpha-bungarotoxin
Detection method/readout

Fluorescence microscopy
Colors

Blue, Green, Orange, Red, Far-red, Near-infrared
Storage Conditions

Store at -10 to -35 °C, Protect from light

Technology

Cellular Stains
CF® Dye & Other Bioconjugates
CF® Dyes
Neuronal Probes and Tools

Tech Tips

Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Battling Tissue Autofluorescence
Tech Tip: Avoiding Artifacts from UV Photoconversion of DAPI and Hoechst
Protocols
SDS
Supporting Documents

Product Description

Alpha-bungarotoxin is a polypeptide snake toxin that binds to the nicotinic acetylcholine receptor found at the neuromuscular junction with high affinity. Fluorescent alpha-bungarotoxin can be used for labeling of nicotinic acetylcholine receptors at neuromuscular junctions in tissue sections. Alpha-bungarotoxin may also be used for detection of GABA A receptor subsets in cells (1), or for labeling recombinant proteins that express the alpha-bungarotoxin binding site (BBS) epitope tag (2).

  • Choose from 9 bright and stable CF® Dye colors or other labels
  • Dye options for super-resolution and 2-photon imaging

We also offer Biotin-XX-alpha-bungarotoxin (catalog. no. 00017), and unconjugated alpha-bungarotoxin.(catalog no. 00010-1). See our complete selection of alpha-Bungarotoxins below.

Superior CF® Dyes

Biotium’s next-generation CF® dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

Super-Resolution Microscopy

Many CF® Dyes are compatible with super resolution imaging, CF® Dyes give the best performance for multiple methods. The superior brightness, photostability, and photochemical switching properties of certain CF® Dyes are ideal for 3-D SIM, 3-D STORM, and other super-resolution and single-molecule imaging approaches. Learn more about CF® Dyes for super-resolution microscopy.

α-Bungarotoxin, CF® Dye and Other Conjugates

Conjugation Ex/Em Size Catalog No. Dye Features
Unconjugated N/A 1 mg 00010-1
Biotin-XX N/A 0.5 mg 00017
CF®405S 411/431 nm 100 ug 00002-100ug CF®405S Features
0.5 mg 00002
CF®488A 490/516 nm 100 ug 00005-100ug CF®488A Features
0.5 mg 00005
CF®543 543/563 nm 100 ug 00026-100ug CF®543 Features
0.5 mg 00026
CF®555 554/568 nm 100 ug 00018-100ug CF®555 Features
0.5 mg 00018
CF®568 562/584 nm 100 ug 00006-100ug CF®568 Features
0.5 mg 00006
CF®594 593/615 nm 100 ug 00007-100ug CF®594 Features
0.5 mg 00007
CF®633 629/650 nm 100 ug 00009-100ug CF®633 Features
0.5 mg 00009
CF®640R 642/663 nm 100 ug 00004-100ug CF®640R Features
0.5 mg 00004
CF®680R 680/701 nm 100 ug 00003-100ug CF®680R Features
0.5 mg 00003
Fluorescein
(FITC)		 498/517 nm 0.5 mg 00011
10 x 50 ug 00013
Tetramethylrhodamine
(TRITC)		 552/578 nm 0.5 mg 00012
10 x 50 ug 00014
Sulforhodamine-101
(Texas Red®)		 595/613 nm 0.5 mg 00015
10 x 50 ug 00016

 

CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.

References

  1. PNAS, 103, 13, (2006), DOI: 10.1073/pnas.0600847103
  2. Meth. Enzymol., 521, (2013), DOI: 10.1016/B978-0-12-391862-8.00006-5
  3. Sci Adv, 6, 15, (2020), DOI: 10.1126/sciadv.aax8382
  4. Am J Physiol Cell Physiol, (2020) DOI: 10.1152/ajpcell.00453.2019
  5. Biochem Biophys Res Commun., 523, 214, (2020), DOI: 10.1016/j.bbrc.2019.12.011
  6. Front Cell Dev Biol., 8, 15, (2020), DOI: 10.3389/fcell.2020.00015
  7. Biophysics, 64, 772, (2019), DOI: 10.1134/S0006350919050129
  8. Cell Physiol Biochem, 53, 701, (2019), DOI: 10.33594/000000166
  9. Biochemistry (Mosc.), 4, 1085, (2019), DOI: 10.1134/S0006297919090116
  10. Biomaterials, 225, 119537, (2019), DOI: 10.1016/j.biomaterials.2019.119537
  11. ACS Chem. Biol., 13, 2568, (2018), DOI: 10.1021/acschembio.8b00513
  12. Cell Mol Neurobiol, 37, 1443, (2017), DOI: 10.1007/s10571-017-0475-3
  13. Neuroscience, 174, 234, (2011), DOI: 10.1016/j.neuroscience.2010.11.016
  14. Am J of Pathol, 177, 2509 (2010), DOI: 10.2353/ajpath.2010.100243
  15. Cell Tissue Biol, 4, 258, (2010), DOI: 10.1134/S1990519X10030077
  16. Neuroscience, 174, 234, (2009), DOI: 10.1016/j.neuroscience.2010.11.016
  17. J Cell Biol, 150, 1385, (2000), DOI: 10.1083/jcb.150.6.1385
  18. Neuron, 23, 675, (1999), DOI: 10.1016/s0896-6273(01)80027-1
  19. Neuron, 12, 167, (1994), DOI: 10.1016/0896-6273(94)90161-9
  20. J Cell Biol, 125, 661, (1994), DOI: 10.1083/jcb.125.3.661
  21. J Biol Chem, 268, 25108, (1993), PMID: 8227074
  22. Muscle Nerve, 5, 140, (1982), DOI: 10.1002/mus.880050211
  23. PNAS 77, 4823, (1980), DOI: 10.1073/pnas.77.8.4823
  24. Science, 196, 540, (1977), DOI: 10.1126/science.850796

Download a list of CF® dye references.

 

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