Alpha 1 Fetoprotein antibody
Cat# orb48271-200ul
Size : 200ul
Brand : Biorbyt
Item 1 of 2
Item 1 of 2
Alpha 1 Fetoprotein antibody
Catalog Number: orb48271
| Catalog Number | orb48271 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Alpha 1 Fetoprotein |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 360-609 of human Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) (NP_001125.1). |
| Concentration | 1 mg/ml |
| Dilution range | WB: 1:500-2000, IF/ICC: 1:50-200 |
| Conjugation | Unconjugated |
| MW | 69kDa |
| UniProt ID | P02771 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Alternative names | anti AFP antibody, anti Alpha fetoglobulin antibod |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of Hep G2 cells using Alpha-Fetoprotein (AFP) Rabbit pAb (orb48271) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates, using Alpha-Fetoprotein (AFP) Rabbit pAb (orb48271) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.