Alpha 1 Fetoprotein antibody
Cat# orb48271-100ul
Size : 100ul
Brand : Biorbyt
Item 1 of 2
Item 1 of 2
Alpha 1 Fetoprotein antibody
Catalog Number: orb48271
Catalog Number | orb48271 |
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Category | Antibodies |
Description | Rabbit polyclonal antibody to Alpha 1 Fetoprotein |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 360-609 of human Alpha-Fetoprotein (Alpha-Fetoprotein (AFP)) (NP_001125.1). |
Concentration | 1 mg/ml |
Dilution range | WB: 1:500-2000, IF/ICC: 1:50-200 |
Conjugation | Unconjugated |
MW | 69kDa |
UniProt ID | P02771 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
Alternative names | anti AFP antibody, anti Alpha fetoglobulin antibod |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence analysis of Hep G2 cells using Alpha-Fetoprotein (AFP) Rabbit pAb (orb48271) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates, using Alpha-Fetoprotein (AFP) Rabbit pAb (orb48271) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.