Our ALK/NPM1 fusion probe is designed to detect fusions between ALK and NPM1. The probe comes labeled in green and orange, but can be customized to meet your needs.
Gene Background: Activation of the tyrosine-kinase domain (TKD) of anaplastic lymphoma kinase (ALK) drives malignant growth in several cancers via oncogenic fusions resulting from chromosomal rearrangements. Approximately 60-85% of anaplastic large-cell lymphoma (ALCL) and 5% of non-small-cell lung cancer (NSCLC) harbor ALK rearrangements.1,2 NPM1 is a ubiquitously expressed nucleolar protein that is involved in ribosomal component shuttling between the cytoplasm and nucleus.3 This translocation yields an 80-kD NPM-ALK chimeric protein that contains the oligomerization motif of NPM fused to the cytoplasmic portion of ALK with an intact kinase domain.3 The protein, which is constitutively activated through autophosphorylation, contributes to malignant cell transformation by activating downstream effectors like STAT3.3 The fusion occurs in up to 50% of anaplastic large cell lymphoma (ALCL) cases, and is more prevalent in pediatric ALCL (83%) than adult ALCL (31%).3,4
** This product is for in vitro and research use only. This product is not intended for diagnostic use.
Gene Summary
This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]
Gene Details
Gene Symbol: ALK
Gene Name: ALK Receptor Tyrosine Kinase
Chromosome: CHR2: 29415639-30144477
Locus: 2p23.2-p23.1