Reagents and instruments for immunology, cell biology and molecular biology.

Aldehyde Dehydrogenase (ALDH) Fluorometric Activity Assay Kit

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Cat# E-BC-F044-96T

Size : 96T

Brand : Elabscience

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Detection Principle	The detection principle of this kit is that the substrate under the action of aldehyde dehydrogenase transforms NAD⁺ into NADH, NADH reacts with the fluorescent probe to form fluorescent substance under the action of enzymes. The activity of ALDH can be calculated by measuring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
Synonyms	ALDH
Sample Type	Serum,Plasma,Animal tissue,Cell
Detection Method	Fluorometric method
Detection Instrument	Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Research Area	Liver And Renal Function
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.06 μmol/L
Detection Range	0.06-3 μmol/L
Precision	inter-assay CV: 8.4% \| intra-assay CV: 0.04
Assay Time	20 min

Cat.No.	Product Name	Sizes
E-BC-K763-M	Alcohol Dehydrogenase (ADH) Activity Assay Kit	96T , 48T
E-BC-K891-M	Ethanol Colorimetric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Rat liver tissue homogenate	150-160
10% Mouse kidney tissue homogenate	90-150
10% Mouse liver tissue homogenate	150-180
10% Mouse lung tissue homogenate	60-90
10% Mouse brain tissue homogenate	30-40
10% Mouse spleen tissue homogenate	60-150
Mouse plasma	3-5
Jurkat cell	8-12

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.