Ac-DEVD-AFC [201608-14-2]
Cat# HY-P1005-1mg
Size : 1mg
Brand : MedChemExpress
Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm).
For research use only. We do not sell to patients.
Custom Peptide Synthesis
Ac-DEVD-AFC Chemical Structure
CAS No. : 201608-14-2
This product is a controlled substance and not for sale in your territory.
Based on 6 publication(s) in Google Scholar
Description |
Ac-DEVD-AFC is a fluorogenic substrate (λex=400 nm, λem=530 nm). |
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In Vitro |
After incubation with Ac-DEVD-AFC for 1 hour, significant increase of caspase-3 activity is observed at 4 hour compare with control. There are no significant increases of caspase-3 activity in Photofrin and LPLI group. The cleavage of Ac-DEVD-AFC in response to caspase-3 activation is remarkably inhibited by shRNA-BimL transfection[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
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Molecular Weight |
729.61 |
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Formula |
C30H34F3N5O13 |
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CAS No. |
201608-14-2 |
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Appearance |
Solid |
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Color |
Light yellow to yellow |
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Emission (Em) |
500 |
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Excitation (Ex) |
380 |
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Sequence |
N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoroMethylcoumarin |
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Sequence Shortening |
Ac-DEVD-7-amido-4-trifluoroMethylcoumarin |
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Shipping | Room temperature in continental US; may vary elsewhere. |
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Storage |
Sealed storage, away from moisture and light
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light) |
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Solvent & Solubility |
In Vitro:
DMSO : ≥ 50 mg/mL (68.53 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O : < 0.1 mg/mL (insoluble) *"≥" means soluble, but saturation unknown. Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO
+
%
+
%
Tween-80
+
%
Saline
The co-solvents required include: DMSO,
. All of co-solvents are available by MedChemExpress (MCE).
, Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration:
mg/mL
Method for preparing stock solution:
mg
drug dissolved in
μL
DMSO (Stock solution concentration: mg/mL).
*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take
μL DMSO stock solution, add
μL .
μL , mix evenly, next add
μL Tween 80, mix evenly, then add
μL Saline.
If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
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Purity & Documentation |
Purity: 98.62% |
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References |
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Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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DMSO | 1 mM | 1.3706 mL | 6.8530 mL | 13.7060 mL | 34.2649 mL |
5 mM | 0.2741 mL | 1.3706 mL | 2.7412 mL | 6.8530 mL | |
10 mM | 0.1371 mL | 0.6853 mL | 1.3706 mL | 3.4265 mL | |
15 mM | 0.0914 mL | 0.4569 mL | 0.9137 mL | 2.2843 mL | |
20 mM | 0.0685 mL | 0.3426 mL | 0.6853 mL | 1.7132 mL | |
25 mM | 0.0548 mL | 0.2741 mL | 0.5482 mL | 1.3706 mL | |
30 mM | 0.0457 mL | 0.2284 mL | 0.4569 mL | 1.1422 mL | |
40 mM | 0.0343 mL | 0.1713 mL | 0.3426 mL | 0.8566 mL | |
50 mM | 0.0274 mL | 0.1371 mL | 0.2741 mL | 0.6853 mL | |
60 mM | 0.0228 mL | 0.1142 mL | 0.2284 mL | 0.5711 mL |
Ac-DEVD-AFC Related Classifications
- Peptides
- Peptide and Derivatives
- Inhibitors and Substrates
- Enzyme Substrates
- Cancer
- Cancer Metabolism and Metastasis
- Others
- Fluorescent Dye
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
Ac-DEVD-AFC201608-14-2Fluorescent DyeInhibitorinhibitorinhibit