Reagents and instruments for immunology, cell biology and molecular biology.
 
> 5× SDS Loading Buffer

5× SDS Loading Buffer

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Cat# E-BC-R288-2

Size : 2mL

Brand : Elabscience

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Product Introduction This product is a protein loading buffer for 5× SDS Loading Buffer electrophoresis.
The main components include SDS, bromophenol blue, reducing agent, etc. Mixed in proportion with proteins at heating can make proteins carry a large number of negative charges and eliminate their own charge differences; at the same time, the hydrogen bonds and disulfide bonds within and between molecules of proteins are disconnected, and the secondary and tertiary structures are destroyed, thereby controlling the migration rate of proteins only related to their molecular weight.
This product adopts improved reductant to replace DTT with irritating odor and toxicity, which is mild and safe with better performance.
Applications WB
Storage Store at -20°C for 12 months; Store at 2-8°C for 6 months.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
Cautions 1.When this product is stored at -20℃, precipitation may occur and must be completely melted before use. If insoluble substances such as precipitate are found, they can be heated in a 50℃ water bath for about 5-15 minutes until completely melted. Store the product in separate containers according to usage and not to perform repeated freeze-thaw treatment.

2.If the temperature is too high (e.g., more than 100℃) or too long (e.g., more than 15 min) when the mixed sample is heated in a water bath, it may cause protein degradation or abnormal color of the indicator in the loading buffer.

3.When using this product, it may become viscous, adhere to the walls, and not easily adsorb, which are all normal phenomena.

4.This product is suitable for denatured polyacrylamide gel electrophoresis.

5.This product is for professional scientific research only.

6.For your safety and health, please wear a lab coat and disposable gloves.
Cat.No. Product Name Sizes
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Other Formats

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® Violet 450

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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