Product Description  

2 × Rapid Taq Master Mix contains Taq DNA Polymerase, elongation promoting factor, dNTP and an optimized buffer system. The amplification speed of this product can reach 15 sec/kb, which is suitable for rapid PCR. The maximum amplification speed within 1 kb can reach 1 sec/kb, greatly saving reaction time. The pre-prepared 2 × Master Mix only needs to add primers and templates to perform amplification, which reduces pipetting operations and improves detection throughput and results reproducibility. The kit has excellent amplification performance and high storage stability. It is suitable for PCR amplification within 5 kb using genome as template and PCR amplification within 10 kb using plasmid and λDNA as template. The protective agent added to the system allows 2 × Master Mix to maintain stable activity after repeated freezing and thawing. This product contains loading buffer and tracking dye, so PCR products can be directly loaded for electrophoresis after the reaction, which is convenient to use. The PCR product has an adenine at the 3' end that can be cloned into the T vector. It is compatible with ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C116/C603).

 Features  

• Rapid amplification: 15 sec/kb; extension speed up to 1 sec/kb (≤ 1kb fragments)

• Convenience: Include tracking dye, direct loading of PCR product on gel

• Good stability: Stable performance after 50 freeze-thaw cycles

• Ready-to-use: Simplified workflow and minimized pipetting steps with 2 × Master Mix format

 Components  

 Storage  

Store at -30 ~ -15°C and transport at ≤0°C. It can be stored at 4°C for 3 months after thawing.