2-Aminoethyl Methanethiosulfonate, Hydrochloride (MTSEA-Chloride)

Reacts specifically and rapidly with thiols to form mixed disulfides. Used to probe the structures of the ACh receptor channel of the GABA receptor channel and of lactose permease. Useful for mapping the pore-lining regions of the ryanodine receptor.||Synonyms:|Methanesulfonothioic Acid S-(2-Aminoethyl) Ester Hydrochloride; MTSEA-Chloride||CAS No:|37597-96-9||Molecular Formula:|C₃H₁₀ClNO₂S₂||Molecular Weight:|191.70||Purity:|≥98%||Appearance:|Off-White to pale beige solid||Melting Point:|As Reported||Solubility:|DMSO, Methanol||Method for Determining Identity:|Proton NMR Spectroscopic and Mass Spectrometric Analysis||TLC Conditions:|As Reported|C18; Methanol: Water: Acetic acid=9:1:0.5; Visualized with Ninhydrin and KMnO4; Single spot, Rf=0.55||Chloride Content:|~19%||Atmosphere:|Inert Gas||Storage and Stability:|May be stored at RT for short-term only. Long-term storage is recommended at -20°C. Stable for 6 months after receipt. Store under inert atmosphere.||At pH 7.5 and room temperature, MTSEA hydrolyzes with a half-life of about 15 minutes, MTSET hydrolyzes with a half-life of 10 minutes, and MTSES hydrolyzes with a half-life of 20 minutes. Routinely, one can use 2.5mM MTSEA, 1mM MTSET, or 10mM MTSES, applied for 1 to 5 minutes. (MTSET is 2.5 times as reactive with small sulfhydryl compounds as is MTSEA, and 10 times as reactive as MTSES). (Stauffer and Karlin, 1994; Karlin, personal communication).||For application to Xenopus Oocytes, dissolve the compounds in 115mM NaCl, 2.5mM KCl, 1.8mM MgCl2, 10mM Hepes, pH 7.5 with NaOH. MTS reagents decompose in buffer very quickly.||•DMSO is a good solvent for the MTS reagents which are not water soluble (i.e. the non-charged MTS reagents).||Important Note: |This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. ||Toxicity and Hazards: |All products should be handled by qualified personnel only, trained in laboratory procedures.