β-Amylase Activity Assay Kit
Cat# E-BC-K005-M-500
Size : 500Assays
Brand : Elabscience
| Detection Principle | The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance. β-amylase was inactivated by the property of amylase not to be heat-resistant, and then the enzyme activity of total amylase and α-amylase is determined. So the activity of β-amylase can be calculated indirectly. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (535-540 nm, optimum wavelength: 540 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.97 U/g tissue |
| Detection Range | 0.97-34.74 U/g tissu |
| Precision | inter-assay CV: 3.2% | intra-assay CV: 2.3% |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 1% Epipremnum aureum tissue homogenate | 1 |
| 1% Green pepper tissue homogenate | 1 |
| 1% Corn grain tissue homogenate | 1 |
| 1% Daucus carota tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Other Clones
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor®405
Elab Fluor®430
Elab Fluor®488
Elab Fluor®568
Elab Fluor®594
Elab Fluor®647
Elab Fluor®680
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Elab Fluor®Violet 450
FITC
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